The relation among myocardial iron load and the severity of heart failure

Abstract Heart failure (HF) symptoms are related to reduced circulatory perfusion, peripheral congestion, also impaired muscle strength & oxygen utilization due iron deficiency. Despite many premises, the correlation between myocardial load and failing heart function was not proved. The purpose of this study assess itself iron-related proteins in human (FH) vs non-failing (NFH) myocardium. group consisted 58 explanted FHs control 31 NFHs unsuitable for transplantation. HF presented with LV dilatation (LVDD 67±8 mm; LVSD 59±13 mm), contraction (LVEF 20±7.5%), RV enlargement (RVD 42±6.5 pulmonary hypertension (2.21±0.93 Wu) elevated NTproBNP (3955.5±2250.5pg/ml). We assessed serum markers: ferritin (FR), transferrin/saturation (TSAT), transferrin receptor (soluble sTfR), sTFR/log FR; homeostasis assessment: M-Iron (Instrumental Neutron Activation Analysis, μg/g) gathering (by ELISA): divalent metal transporter (DMT-1); L-type calcium channel (L-CH), TfR-1/TfR-2; ferritins: heavy (FT-H), light (FT-L) chain, mitochondrial (FT-MT); ferroportin (FPN) oxidative stress marker: 4-hydroxynonenal (4-HNE). In FH NFH, (164.6±25.2 182.3±23.9; p=0.0358) however, did apply entire group. Therefore we divvied into two subgroups (based on concentration NFH controls). Average concertation non-iron-deficient (NIDM) subgroup 172.5±19.1 (n=35) iron-deficient (IDM) wad 127.0±16.5; (n=23, p=0.0001). Regardless expressions were reduced: L-CH (NIDM IDM NFH: 49.2±9.2; 42.5±4.2; 67.3±23.0, p=0.0022); FT-L (16.4±7.3; 11.6±4.8; 26.3±7.5, p= <0.0001, respectively); FT-MT (41.2±7.6; 38.7±4.1; 59.3±10.4 p≤0,0001, respectively). With regard load, only DMT-1 expression changed 4.8±0.7; 4.0±0.5; 5.8±0.8; p≤0.0001). 4-HNE levels increased, regardless (NIDM-Iron IDM-Iron NFH; 55.7±29.2; 57.2±47.8; 9.9±8.1 Interestingly, IDM-Iron) modify clinical parameters. commonly used systemic markers FR proved difference 0.6±0.2; 0.7±0.3; p=0.047910) FH, observed, load. observed reduction independent except main transportation protein cell. results showed that degree unlikely be affected by protein. from increased whole Commonly define Funding Acknowledgement Type funding sources: Public Institution(s). Main source(s): Cardinal Stefan Wyszyński National Institute Cardiology